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Manual Microinjector

The Manual Injector is a manual syringe driver for pneumatic or hydraulic control of injection needles or holding pipettes. It is suitable for injecting volumes in the nanoliter to microliter range. It is also widely used as a fluid control device for applications requiring sensitive manual control of the displacement of microneedle contents. The system is constructed from the highest quality parts. The base assembly is black anodized aluminum. A non-rotating Mitutoyo micrometer provides the drive to the gas-tight syringe1. A precision 3-way valve provides a convenient method for filling the fluid line and clearing air bubbles from the line. Teflon tubing, chromatography connectors and a pipette holder2 complete the system.

The injection resolution is dependent on the volume of the syringe that is installed, as outlined in the table below. For example, with a 25µL syringe installed, one complete rotation of the micrometer (25 divisions) yields a displacement equivalent to a volume of 267nL and turning the micrometer one division (0.001 in) yields 10.6nL.

Syringe
Volume
Volume Per
Revolution
Volume Per
Division
10µL
106nL
4.2nL
25µL
267nL
10.6nL
50µL
529nL
21.2nL
100µL
1.06µL
42.3nL
250µL
2.65µL
105.8nL
500µL
5.29µL
211.7nL
1000µL
10.58µL
423.4nL

1 Please specify the syringe volume that you will be using. For animal IVF, CRISPR, and Pronuclear Injection Holding applications, a 500µL or 1000µL syringe is recommended. The 10μL syringe will require a special assembly and parts if ordered separately.
2 Unless specified otherwise, a pipette holder for 1mm outside diameter glass will be supplied. The diameter of the pipette holder is 0.25in / 6.4mm. For micromanipulators that connot accept this size holder an adapter is optionally available. Please contact Sutter for further information.

Manual Injector

Dimensions
12 in x 3.75 in x 4 in | 30 cm x 9.5 cm x 10 cm

Weight
2.5 lbs | 1 kg

 

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Part Number > MANUAL MICROINJECTOR
International prices vary by country. Please contact your local distributor or Sutter Instrument for a quotation.

Part Number Description
MANUAL Manual injector with non-rotating micrometer, 3-way valve, precision 50µL gas-tight syringe, teflon tubing, connectors and MI-10010 pipette holder assembly for 1.0mm capillary tubing1

1 Other syringe volumes, and pipette holder sizes are available upon request


ACCESSORIES

Part Number Description
MI-10010 Pipette holder assembly for 1.0mm OD glass
MI-10012 Pipette holder assembly for 1.2mm OD glass
MI-10015 Pipette holder assembly for 1.5mm OD glass
BR-OIL Oil for injector (100ml)
V200050 Teflon tubing
V0011801 10µL gas-tight syringe
V001181 25µL gas-tight syringe
V001182 50µL gas-tight syringe
V001183 100µL gas-tight syringe
V001184 250µL gas-tight syringe
V001185 500µL gas-tight syringe
V0011861 1000µL gas-tight syringe
V300450 1.0 - 1.2mm O-Ring (Package of 6)
V300455 1.5mm O-Ring (Package of 6)
V001104 1/16 inch clear ferrule for tubing

1 Requires special assembly and parts when ordered separately. Please contact Sutter.

Applications

  • Pronuclear Injection
  • Generation of holding force
  • Nuclear transfer
  • Preimplantation genetic testing
  • Embryonic stem cell injection
  • Xenopus oocyte injection
  • Embryo injections
  • Polar body biopsy
  • Blastocyst injection
  • Single-cell picking

 

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