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3-axis / Narrow-Format Micromanipulator System

TRIO™ MPC-165 System

The MPC-165 System consists of the MPC-100 controller and an MP-865 narrow-format micromanipulator.

Our new MP-865 "narrow format" mechanical is designed specifically for patch-slice work that require multiple pipettes, as well as for other setups where space is limited. We designed the MP-865 to minimize the width, allowing as many manipulators to be grouped together as possible. Travel in the Y-axis is shortened (12.5 mm) because radially oriented manipulators do not require long travel in the traverse axis. Travel in the X-axis has been increased to 50 mm to facilitate fast and easy pipette exchange. Built with the same precision cross-roller bearings and lead-screw as the MP-845 design.

The optional narrow-format tower with linear slide, or rotating base, can be added for mounting the MP-865 mechanical. With the 150 mm throw of the linear slide, the manipulator can be quickly and smoothly retracted out to a location where there is easy access to the pipette for replacement. A rotating base can be used for mounting to large platform stages like the Sutter MT-78, or smaller Sutter MD Series platforms.

The compact design of the TRIO MPC-100 integrated Rotary Optical Encoder (ROE) controller requires minimal bench space; provides quiet, fan-free operation; and is easy to use. No rack mounted controller is required. Position coordinates, in relative or absolute values, are displayed directly on the ROE. The TRIO manipulators use a logarithmic acceleration algorithm that eliminates the need for speed selection. As the knobs on the ROE are turned faster, acceleration ramps up. This allows for smooth and intuitive motion control of electrode position without the need to stop and change speeds or lift your hand from the knobs. A Y-axis lockout function (accessible by DIP switch) is also available, allowing X/Z-only axial movement during HOME and WORK repositioning. Each MPC-100 can control up to two MP-865 manipulators

Five conveniently located buttons control all the functions you will need in normal operation. Press and hold the [WORK] button to quickly store a work position; pressing [WORK] after this will return the manipulator to the same location. [HOME] sends the manipulator to a second position, often set for a point furthest from the microscope, which is useful for rapid pipette exchange. Pressing [SPEED] allows the selection of one of 4 speed ranges. With practice, there is no need to ever change speeds, however, we have included three low speed ranges for those who work at very high magnification. Holding [SPEED] for three-seconds will lock the knobs out, to prevent accidental movement. Display coordinates can toggle between relative and absolute by pressing the [RELATIVE] button; holding the button down will zero the relative coordinates. Finally, [PULSE] activates a pulse movement mode that produces small, rapid bursts of motion that can be advantageous for cell penetration with sharp electrodes. Hold [PULSE] for three-seconds to set or modify the 4th axis angle between 0 and 90 degrees.

Designed with maximum flexibility in mind, a DIP switch on the controller changes the directional movement of the ROE knobs to accommodate the preference of the user. The TRIO comes standard with a universal mounting system suitable for the most popular headstages or pipette holders.

trio mpc-165
TRIO MPC-100 Controller
  • Controls one or two manipulators
  • Sub-micron (less than 100 nm) resolution
  • User-selectable angle from 0 - 90 degrees via ROE input
  • Fast movement with a top speed of 3 mm/sec (while homing)
  • Compact, fanless, user-friendly ROE controller preserves bench and rack space
  • Push button control of multiple functions - WORK, HOME, LOCK, PULSE, and RELATIVE
  • DIP switches on ROE select direction of movement produced by turn of ROE knob
  • USB interface for computer control

MP-865 Mechanical

  • Mechanically robust construction for high stability
  • Precision cross-roller bearings
  • Three independent axes - 50 mm travel in X,  12.5 mm in Y, and 25 mm in Z
  • Carries up to a kilogram
  • Suited for in vitro and in vivo electrophysiological recording
  • Universal mounting system for headstage or pipette holder
  • Optional MT-73 narrow stand with linear slide and built in rotating base

Resolution and Travel
Minimal microstep size is 46.88 nanometers per microstep
Display has single micron resolution
Full travel is 50 mm in X, 12.5 mm in Y, and 25 mm in Z

Maximum Speed
3 mm/second

Long Term Stability
< 1.0 micron in 4 hours

TRIO MPC-100 Control Box
6.25 in x 8 in x 5.9 in | 15.9 cm x 20.3 cm x 10.2 cm

Manipulator Weight
MP-865 (aluminum)
3.5 lbs | 1.6 kg

TRIO MPC-100 Controller
2.3 lbs | 1.04 kg

Electrical
115/230 Volts
50/60 Hertz power line

Part Number > TRIO MPC-165 Systems
International prices vary by country. Please contact your local distributor or Sutter Instrument for a quotation.

Part Number Description
 
MPC-165 System includes one TRIO™ MPC-100 controller, one MP-865/M manipulator mechanical, mounting adapter plate, rod holder, 4" dovetail extension, cables, power cord and manual.*
 
MPC-165-2 MPC-165 with two MP-865/M mechanicals. (please specify right or left handed)
 
 

 


COMPONENTS

Part Number Description
 
 
MP-865-ML The MP-865 (left handed) manipulator mechanical alone. Includes mounting adapter plate, rod holder, 4 inch dovetail extension, and cable to connect manipulator to controller.
MP-865-MR The MP-865 (right handed) manipulator mechanical alone. Includes mounting adapter plate, rod holder, 4 inch dovetail extension, and cable to connect manipulator to controller.
   

MOUNTING SYSTEMS

MT-1004 Systems
Includes the MT-500 manual X-Y translator, MT-150 chamber column, two MT-74 micromanipulator columns (without linear slide)

Part Number Description
MT-1004/Y511 MT-1004 for the Olympus BX51WI
MT-1004/Y531 MT-1004 for the Olympus BX53/63
MT-1004/Y711 MT-1004 for the Olympus IX71
MT-1004/Z251 MT-1004 for the Zeiss Axioskop 2 FS
MT-1004/Z451 MT-1004 for the Zeiss Axio Examiner
MT-1004/N651 MT-1004 for the Nikon FN1
MT-1004/L301 MT-1004 for the Leica DMLFS
MT-1004/L351 MT-1004 for the Leica DM6000 FS

MT-2004 Systems
Includes the MT-800 motorized X-Y translator, MP-285 controller and ROE, MT-150 chamber column, two MT-74 micromanipulator columns (without linear slide), cables, and manual

Part Number Description
MT-2004/Y511 MT-2004 for the Olympus BX51WI
MT-2004/Y51/FD1,2 MT-2004 with focus drive for the Olympus BX51WI
MT-2004/Y531 MT-2004 for the Olympus BX53/63
MT-2004/Z251 MT-2004 for the Zeiss Axioskop 2 FS
MT-2004/Z451 MT-2004 for the Zeiss Axio Examiner
MT-2004/N651 MT-2004 for the Nikon FN1
MT-2004/L301 MT-2004 for the Leica DMLFS
MT-2004/L351 MT-2004 for the Leica DM6000 FS

1 Please specify chamber type when ordering
2 Please contact Sutter for availability of focus drive on other microscope makes & models


CHAMBER COLUMNS

Part Number Description
MT-150/W20 Chamber column for Warner Series 20 chamber (octagon)
MT-150/W20R Chamber column for Warner Series 20 chamber (round)
MT-150/ALA35 Chamber column for ALA 35mm chamber
MT-150/ALA-MS Chamber column for ALA MS
MT-150/CUST Chamber column for non-standard recording chamber
MT-150/PT Chamber column for petri dish or lid
MT-150/AUTO Chamber column for AutoMate round stage adapter

ACCESSORIES

Part Number Description
MT-731 Narrow-format stand with linear slide
MT-741 Narrow-format stand (no linear slide)
MAG-MT74 Magnetic feet for MT-74 (set of two)
265RBI2 Rotating base
M100106 Flat side panel for MPC-200 controller (each)
MP-RISER-0.53 1/2 inch riser
MP-RISER-1.03 1 inch riser
MP-CLIP4 Rod holder (for rod OD 6.25 mm or larger)
MP-ROD Rod holder (for rod OD 6.25 mm or larger)
EHOLDER Micropipette holder - 1.0-1.5 mm glass

1 Suitable for upright microscopes.
2 Useful with Sutter MD stand and large platform stages such as the MT-78-FS, MP-78 and MPC-78.
3 Risers can be combined to achieve desired height.
4 Suitable for multielectrode probes.

APPLICATIONS

  • Multi-electrode recording
  • Auditory research
  • Whole-cell recordings
  • Dispersed cell line studies
  • Network studies
  • Single-channel recordings
  • Tissue slice recordings
  • Pronuclear microinjection
  • Embryonic stem cell transfer
  • Somatic cell nuclear transfer (ES cell)
  • Piezo-assisted sperm-mediated transgenesis

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