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BOB
Open-Design Upright Microscope

The Sutter BOB — designed to eliminate the conventional microscope frame—is a simple, open-design upright microscope platform ideal for slice electrophysiology, wide field functional imaging, two photon retinal imaging, photostimulation and new techniques just being developed! A microscope, in its simplest form, is an objective and a tube lens. Other components of most modern microscopes are designed to serve specific functions: different types of experiments, methods of illumination and means of signal detection.

Replacing the microscope frame with an optical rail builds in the ability to adjust the overall height of the microscope, unheard of in conventional microscope designs. Work on slices in January, do in vivo experiments in March. The BOB microscope is a compact, single assembly that mounts to the “blue rail” with one massive, stable connection. Focusing is motorized and incorporated between the focus arm and the optical rail.

Fluorescence epi-illumination is built into the basic BOB via an Olympus vertical illuminator. LED transmitted light illumination uses the Olympus Oblique Coherent Contrast (OCC) condenser. Sutter’s TLED and TLED controller form the trans-illumination light source. The TLED controller is capable of being triggered with a digital signal eliminating the need for shutters and adding the ability to photostimulate from the trans location. In experiments where transmitted light is not desired, the LED, condenser focus mechanism and OCC condenser are easily removed as a single assembly. Additionally, the transmitted light path is shorter than in other frames, allowing the microscope body to sit significantly lower than a conventional microscope. When the microscope is shorter, there is more stability, increased ergometrics and ease of use.

The Sutter BOB when configured with an optional motorized XY stage or translator with MPC-200 controller, takes full advantage of our free Multi-Link™ software program for micromanipulator positioning. During whole-cell patch recording in slices, it is common to search a large area of the slice to find appropriate neurons. If the BOB is configured with Multi-Link, after you find your target, Multi-Link will then retrieve your recording and stimulation pipettes to the same field of view so that you can begin recording immediately. If later you need to stimulate a region outside the current field of view, Multi-Link can release the recording pipette and allow you to reposition the objective and stimulating pipette(s) to the new stimulation region.

    APPLICATIONS
  • in vivo and in vitro
  • Whole-cell imaging
  • Intracellular imaging
  • Material science
    FEATURES
  • Optional motorized fixed XY stage or motorized translator
  • Open design microscope with motorized focus
  • Quickly configurable based on experimental needs
  • Optimized to allow in vivo and in vitro experimentation on one setup
  • Designed for use with Olympus objective lenses
  • Free Multi-Link™ software coordinates movement with micropipette positioning of MPC-200
  • Oblique Coherent Contrast (OCC) or Differential Interference Contrast (DIC)
  • Epi-fluorescent illumination

TECHNICAL SPECIFICATIONS

Dimensions
9.1 in x 10.8 in x 20.4 in | 23.1 cm x 27.5 cm x 51.9 cm

Weight
BOB
23.9 lbs | 10.8 kg
BOB-TL
29.3 lbs | 13.3 kg

Electrical
115/230 volts
50/60 Hertz power line


CE
ROHS

CONFIGURE YOUR BOB™ MICROSCOPE

STEP 1
Use this chart to determine the BOB system suited to your requirements


BOB SYSTEMS WITH FLUORESCENCE

CATALOG NUMBER
Trinocular head or
camera mount
Transmitted
light
Add a second wavelength
to transmitted light
BOB-FL Camera No No
BOB-FLTL Camera Yes No
BOB-FLTL2 Camera Yes Yes
BOB-FLTR Trinocular Head No No
BOB-FLTRTL Trinocular Head Yes No
BOB-FLTRTL2 Trinocular Head Yes Yes

BOB SYSTEMS WITHOUT FLUORESCENCE

CATALOG NUMBER
Trinocular head or
camera mount
Transmitted
light
Add a second wavelength
to transmitted light
BOB Camera No No
BOB-TL Camera Yes No
BOB-TL2 Camera Yes Yes
BOB-TR Trinocular Head No No
BOB-TRTL Trinocular Head Yes No
BOB-TRTL2 Trinocular Head Yes Yes

US PRICES > BOB
International prices vary by country. Please contact your local distributor or Sutter Instrument for a quotation. Prices subject to change without notice.

BOB systems with fluorescence
Catalog Number Description Price
BOB-FL BOB microscope with epi-illuminator $ 17,900
BOB-FLTL BOB with epi-illuminator, transmitted light $ 22,400
BOB-FLTL2 BOB with epi-illuminator, dual transmitted light $ 23,800
BOB-FLTR BOB with epi-illuminator, trinocular head $ 24,700
BOB-FLTRTL BOB with epi-illuminator, trinocular head, transmitted light $ 29,200
BOB-FLTRTL2 BOB with epi-illuminator, trinocular head, dual transmitted light $ 30,600

BOB systems without fluorescence
Catalog Number Description Price
BOB BOB microscope $ 12,900
BOB-TL BOB with transmitted light $ 17,400
BOB-TL2 BOB with dual transmitted light $ 18,800
BOB-TR BOB with trinocular head $ 19,700
BOB-TRTL BOB with trinocular head, transmitted light $ 24,200
BOB-TRTL2 BOB with trinocular head, dual transmitted light $ 25,600

STEP 2

CHOOSE YOUR OBJECTIVE(s)

The BOB uses a standard Olympus 2-position nosepiece. You can
use your own objectives or select up to 2 from the choices below.

Objectives highlighted in GREEN are recommended for electrophysiology.

Catalog Number Description Price
OBJ-522 4X-UPLFLN4X;U PLAN FLUORITE 4X OBJECTIVE;NA 0.13, WD 17mm $ 436
OBJ-342 5X-LMPLFLN5X;LWD M PLAN FL 5X OBJECTIVE;NA 0.13, WD 22.5mm $ 946
OBJ-343 10X-LMPLFLN10X;LWD M PLAN FL 10X OBJECTIVE;NA 0.25, WD 21mm $ 1,272
OBJ-524 10X-UPLFLN10X2;U PLAN FLUORITE 10X OBJECTIVE;NA 0.3, WD 10mm $ 779
OBJ-824 10X-UPLSAPO10X2;U PLANS S-APO 10X OBJECTIVE;NA 0.4, WD 3.1mm $ 2,255
OBJ-525 20X-UPLFLN20X;U PLAN FLUORITE 20X OBJECTIVE;NA 0.5, WD 2.1mm $ 925
OBJ-585 20X-UMPLFLN20XW;LWD PLAN FLUOR 20X WTR DIPPING OBJ;NA 0.5, WD 3.5mm, IR $ 1,657
OBJ-766 20X-UAPON20XW340;UAPON 20X WATER 340nm OBJ;NA 0.7, WD 0.35mm $ 4,222
OBJ-825 20X-UPLSAPO20X;U PLAN S-APO 20X OBJECTIVE;NA 0.75, WD 0.6mm $ 2,435
OBJ-527 40X-UPLFLN40X;U PLAN FLUORITE 40X OBJECTIVE;NA 0.75, WD 0.51mm $ 1,012
OBJ-587 40X-LUMPLFLN40X/W;U M PLAN FLN 40X/W OBJ;NA 0.8, WD 3.3mm, IR, ECO $ 2,556
OBJ-769 40X-UAPON40XW340;UAPON 40X WATER OBJ;NA 1.15, WD 0.25mm, W/CC, BFP1 $ 4,222
OBJ-350 50X-LMPLFLN50X;LWD M PLAN FL 50X OBJECTIVE;NA 0.5, WD 10.6mm $ 2,706
OBJ-592 60X-LUMPLFLN60X/W;U M PLAN FLN 60X/W OBJ;NA 1.0, WD 2.0mm, IR, ECO $ 3,007
OBJ-672 60X-LUMFLN60X/W;NA 1.1, WD 1.5mm, 25 ANGLE, IR.CC, ECO $ 9,267
OBJ-832 60X-UPLSAPO60XO;U PLAN S-APO 60X OIL OBJ;NA 1.35, WD 0.15mm, BFP1 $ 5,089
OBJ-893 60X-UPLSAPO60XW;U PLAN S-APO 60X W OBJ;NA 1.2, WD 0.28mm, W/CC 0.13-0.21 $ 9,505

STEP 3
For models with TL in the part number, choose a condenser. For DIC models,
select the condenser that matches the highest power of your selected objectives.


Catalog Number Description Price
OCC Oblique coherent condenser $ 2,300
DIC-40X Differential interference contrast condenser for BOB with 40x objective $ 5,960
DIC-60X Differential interference contrast condenser for BOB with 60x objective $ 6,460
 

STEP 4
For models with FL in the part number, choose a filter cube(s) and a light source.


FILTER CUBES
Catalog Number Description Price
CUBE-GFP Filter Cube - GFP $ 1,220
CUBE-CY3 Filter Cube - CY3 $ 1,220
CUBE-YFP Filter Cube - YFP $ 1,220
CUBE-DAPI Filter Cube - DAPI $ 1,220
CUBE-A635 Filter Cube - ALEXA 635 $ 1,220
 

LIGHT SOURCES
Catalog Number Description Price
HPX-L5 Lambda HPX-L5 High powered LED light source $ 5,650
LS-OF30 Lambda LS with 300 Watt ozone free lamp $ 5,550
LB-421 Lambda 421 Optical Beam Combiner and controller $ 9,715
LB-OBC Lambda OBC Optical Beam Combiner Contact Sutter
FLED-BOB Lambda FLED for BOB $ 2,860
FLED-DC-BOB Dual Channel Lambda FLED for BOB $ 5,825
LG-BOB Light guide adapter $ 580
 

STEP 5
Select a stage and translator as needed.
If you are planning on using our free Multi-Link Software, you
will need to select a stage or translator with the MPC-200 controller


MOTORIZED LARGE PLATFORM STAGE
Catalog Number Description Price
MPC-881 Moving platform stage plate with motorized gantry supports, MPC-200 controller and ROE, cables and manual $ 13,615
MPC-88-M61 Same as MPC-88 except with M6 tapped holes $ 13,615
 
MPB-881 Moving platform stage plate with motorized gantry supports, BOB controller, cables and manual $ 11,025
MPB-88-M61 Same as MPB-88 except with M6 tapped holes $ 11,025
 
MP-881 Moving platform stage plate with motorized gantry supports, MP-285 controller and ROE, cables and manual $ 12,415
MP-88-M61 Same as MP-88 except with M6 tapped holes $ 12,415

1 Please specify chamber type when ordering


MOTORIZED TRANSLATOR AND STANDS
Catalog Number Description Price
MT-2000-BOB Motorized translator with MP-285 controller, ROE, and stands $ 11,580
MT-2200-BOB Motorized translator with MPC-200 controller, ROE, and stands $ 12,025
MT-2800-BOB Motorized translator with BOB controller, ROE, and stands $ 11,580
 
MOTORIZED TRANSLATOR AND FIXED PLATFORM STAGE
Catalog Number Description Price
MT-2088-BOB Motorized translator with MP-285 controller, ROE, and fixed platform stage $ 12,700
MT-2288-BOB Motorized translator with MPC-200 controller, ROE, and fixed platform stage $ 13,255
MT-2888-BOB Motorized translator with BOB controller, ROE, and fixed platform stage $ 11,700
 
MOTORIZED TRANSLATOR ONLY (no stands or platform)
Catalog Number Description Price
MT-800-BOB Motorized translator with MP-285 controller and ROE $ 9,380
MT-820-BOB Motorized translator with MPC-200 controller and ROE $ 9,930
MT-880-BOB Motorized translator with BOB controller and ROE $ 8,380

STEP 6
Select a camera as needed


CAMERA
Catalog Number Description Price
CAM-EL ELECTRO, Cooled 1.4MP CCD Camera $ 4,950
CAM-PR PRIME 25B, Back illuminated CMOS Camera $ 24,950
CAM-BSI PRIME BSI, High-res scientific CMOS Camera $ 18,450
 

A C C E S S O R I E S
MP-88 / MPB-88 / MPC-88 Chamber inserts
Catalog Number Description Price
X0405002 Warner Series 20 (round) - aluminum $ 117
X0405032 Warner Series 20 (round) - stainless steel $ 117
X0405052 Warner Series 20 (octagonal) - aluminum $ 117
X0405072 Warner Series 20 (octagonal) - stainless steel $ 117
X0405102 50mm round - aluminum $ 117
X0405122 50mm round - stainless steel $ 117
X0404972 Blank insert - Aluminum $ 117
X0404982 Blank insert - stainless Steel $ 117
SI-SLIDE2 Insert with slide holder $ 117
SI-PETRI For petri dish and slides $ 329
SI-W302 Warner Series 30 platform - aluminum $ 117
SI-W30ST2 Warner Series 30 platform - stainless steel $ 117
SI-ALA-MS2 ALA MS series insert - aluminum $ 117
SI-CUSTOM Custom stage insert $ 361

2 No charge when ordered with MP-88, MPB-88 or MPC-88

PRODUCT INFORMATION

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