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Capillary Glass & Filaments

Sutter Instrument Company, in addition to the finest micropipette pullers available, offers a wide selection of high quality capillary glasses in various sizes and materials. Though there are many types and sizes of capillary glass available, we have carefully selected only those that pass our criteria. Our expertise in micropipette technology assures you of precision and high quality.

We offer capillary glass tubing in three different compositions; quartz, borosilicate and aluminosilicate. Each composition has its own unique properties and the selection will be determined by your application and your puller’s capabilities.

Fire Polishing
All borosilicate and aluminosilicate capillary glass offered by Sutter Instrument have fire-polished ends. This process eliminates any sharp edges, making it easier to insert into holders, and does not affect the electrical or mechanical properties of the glass.

Custom Pipettes
Sutter Instrument can make custom pipettes and microtools not commonly available from other pipette manufacturers. The custom pipettes are considered non-sterile and are manufactured for research applications and non-human use. Please contact Sutter Instrument for further details.

Sizes
Sutter Instrument capillary tubing is available in a broad range of wall thickness which allows you to select the size necessary for your application. The ratios of inside diameter to outside diameter typically run between 0.5mm and 0.75mm with the lower value being referred to as thick wall tubing and the top of the range as thin wall tubing. All other factors being equal, the thicker wall capillaries produce pipettes with longer tapers and smaller tips which make it more suitable for intracellular microelectrodes. These thicker wall pipettes tend to reduce the noise contributions due to capacitance which makes them more suitable for patch clamp pipettes. Thinner wall tubing allows for larger pore openings which make it ideal for microinjection needles and low resistance microelectrodes.

Filamented Glass - TechNote: What is filamented glass and who needs it?
Filamented glass has a small rod of glass annealed to the inner wall and this rod (filament of glass) creates the capillary action required to back-fill the pipette with solution. If the resulting pipette tip is under 1µl and being used for microinjection or being used for recording, we recommend "filamented-glass". The filament in the glass not only provides capillary action for quick filling of the micropipette, it also helps to reduce the incidence of air bubbles when introducing solution into the pipette.

GLASS COMPOSITION

Borosilicate (CORNING 7740)
The most commonly used glass is borosilicate. Sutter Instrument offers only TYPE I-CLASS A borosilicate as described by ASTM Standard 3.1.2*. This glass softens at 821 degrees Celsius and, as it is pulled, maintains its ratio of inside diameter to outside diameter over the total taper length. Borosilicate softens at a lower temperature than our other glasses and has a wider working range. These unique properties allow for a greater variety of shapes used in microelectrodes, patch pipettes, microinjection needles and, in the case of solid rod, chromosome dissection tools.

Aluminosilicate (SCHOTT 8252)
Aluminosilicate softens at a higher temperature (935 degrees Celsius) than borosilicate and is workable over a much narrower range. It has a tendency to continuously thin out as it is drawn which allows extremely fine tips with very short tapers. For example, we have pulled aluminosilicate tips in the 20-30 nanometer range with taper lengths of 5-6mm. Its resistivity is several orders of magnitude higher than borosilicate, thus reducing leakage currents when used in ion-selective micropipettes. Aluminosilicate is harder than borosilicate which results in a pipette that is more suitable for penetrating tough tissues.

Quartz (HERAEUS HSQ300)
The finest and purest glass available is quartz. It is superior to all other glasses in its mechanical, electrical and optical qualities. It has the lowest dielectric constant, the lowest loss factor and the highest volume resistivity making it ideal for patch clamp recording. Its chemical purity virtually eliminates leakage of ions** and by using quartz in single channel patch clamp recordings the lowest background noise levels have been achieved***. Due to its high melting point, it cannot be pulled on conventional pullers. However, it can be pulled easily with the Sutter CO2 laser-based P-2000.

* ASTM Designation E438-90 - April 1990

** Zuazaga C., Steinacker A. Patch-clamp recording of ion channels: Interfering effects of patch pipette glass. News in Physiological Sciences: International Union of Physiological Sciences and the American Physiological Society: 5:155-158, August 1990

*** Rae, James L., Levis Richard A. A Method for exceptionally low noise single channel recordings. Pflügers Archive; European Journal of Physiology:420:618-620, Springer-Verlag 1992

US PRICES > Glass
International prices vary by country due to associated importation costs. Please contact your local distributor or Sutter Instrument for a quotation. Prices subject to change without notice.

Thick/Standard Wall Borosilicate Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
BF100-50-7.5 1.00mm 0.50mm 7.5cm 225 $57
BF100-50-10 1.00mm 0.50mm 10cm 225 $53
BF100-50-15 1.00mm 0.50mm 15cm 225 $65
BF100-58-10 1.00mm 0.58mm 10cm 250 $52
BF100-58-15 1.00mm 0.58mm 15cm 250 $62
BF120-60-10 1.20mm 0.60mm 10cm 225 $59
BF120-69-7.5 1.20mm 0.69mm 7.5cm 250 $57
BF120-69-10 1.20mm 0.69mm 10cm 250 $59
BF120-69-15 1.20mm 0.69mm 15cm 250 $63
BF150-75-7.5 1.50mm 0.75mm 7.5cm 225 $63
BF150-75-10 1.50mm 0.75mm 10cm 225 $59
BF150-86-7.5 1.50mm 0.86mm 7.5cm 250 $56
BF150-86-10 1.50mm 0.86mm 10cm 250 $53
BF150-86-15 1.50mm 0.86mm 15cm 250 $98
BF200-100-10 2.00mm 1.00mm 10cm 225 $102
BF200-116-10 2.00mm 1.16mm 10cm 250 $110
BF200-116-15 2.00mm 1.16mm 15cm 250 $111

Thick/Standard Wall Borosilicate Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
B100-20-10* 1.00mm 0.20mm 10cm 225 $125
B100-50-10 1.00mm 0.50mm 10cm 225 $54
B100-50-15 1.00mm 0.50mm 15cm 225 $93
B100-58-10 1.00mm 0.58mm 10cm 250 $54
B100-58-15 1.00mm 0.58mm 15cm 250 $56
B120-69-8 1.20mm 0.69mm 8cm 250 $50
B120-69-10 1.20mm 0.69mm 10cm 250 $56
B120-69-15 1.20mm 0.69mm 15cm 250 $54
B150-86-7.5 1.50mm 0.86mm 7.5cm 250 $54
B150-86-10 1.50mm 0.86mm 10cm 250 $58
B150-86-15 1.50mm 0.86mm 15cm 250 $90
B200-116-10 2.00mm 1.16mm 10cm 250 $83
B200-116-15 2.00mm 1.16mm 15cm 250 $92
* The ends are not fire polished. Special order minimum of 2.

Heavy Polished Thick/Standard and Thin Walled Borosilicate Glass1
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
BF150-86-7.5HP 1.50mm 0.86mm 7.5cm 250 $61
BF150-86-10HP 1.50mm 0.86mm 10cm 250 $61
BF150-110-7.5HP 1.50mm 1.10mm 7.5cm 225 $61
BF150-110-10HP 1.50mm 1.10mm 10cm 225 $61

Heavy Polished Thick/Standard and Thin Walled Borosilicate Glass1
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
B100-30-7.5HP 1.00mm 0.30mm 7.5cm 225 $67
B100-50-7.5HP 1.00mm 0.50mm 7.5cm 225 $64
B150-86-7.5HP 1.50mm 0.86mm 7.5cm 250 $61
B150-86-10HP 1.50mm 0.86mm 10cm 250 $63
B150-110-7.5HP 1.50mm 1.10mm 7.5cm 225 $61
B150-110-10HP 1.50mm 1.10mm 10cm 225 $61
1Useful for added protection of gaskets and wire in headstages.

Thin Wall Borosilicate Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
BF100-78-10 1.00mm 0.78mm 10cm 250 $55
BF100-78-15 1.00mm 0.78mm 15cm 250 $64
BF120-94-8 1.20mm 0.94mm 8cm 250 $58
BF120-94-10 1.20mm 0.94mm 10cm 250 $56
BF120-94-15 1.20mm 0.94mm 15cm 250 $67
BF150-110-7.5 1.50mm 1.10mm 7.5cm 225 $52
BF150-110-10 1.50mm 1.10mm 10cm 225 $51
BF150-117-10 1.50mm 1.17mm 10cm 250 $52
BF150-117-15 1.50mm 1.17mm 15cm 100 $51
BF150-120-10 1.50mm 1.20mm 10cm 250 $46
BF165-120-7.5 1.65mm 1.20mm 7.5cm 225 $56
BF165-120-10 1.65mm 1.20mm 10cm 225 $58
BF200-156-10 2.00mm 1.56mm 10cm 250 $64
BF200-156-15 2.00mm 1.56mm 15cm 100 $70

Thin Wall Borosilicate Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
B100-75-10 1.00mm 0.75mm 10cm 225 $54
B100-75-15 1.00mm 0.75mm 15cm 225 $60
B120-90-8 1.20mm 0.90mm 8cm 225 $52
B120-90-10 1.20mm 0.90mm 10cm 225 $52
B120-90-15 1.20mm 0.90mm 15cm 225 $52
B150-110-7.5 1.50mm 1.10mm 7.5cm 225 $52
B150-110-10 1.50mm 1.10mm 10cm 225 $50
B150-117-10 1.50mm 1.17mm 10cm 225 $61
B200-156-10 2.00mm 1.56mm 10cm 250 $55
B200-156-15 2.00mm 1.56mm 15cm 100 $61

Multibarrel Borosilicate Glass*
With Filament
Catalog Number Number of Barrels In/Outside Diameter Overall Length Pieces per Package Price
2BF100-50-10 2 barrels 1.00/0.50 10cm 75 $62
2BF100-75-10 2 barrels 1.00/0.75 10cm 75 $62
2BF150-86-10 2 barrels 1.50/0.86 10cm 100 $67
2BF150-86-15 2 barrels 1.50/0.86 15cm 100 $87
3BF100-50-10 3 barrels 1.00/0.50 10cm 75 $67
3BF100-75-10 3 barrels 1.00/0.75 10cm 75 $67
3BF120-69-10 3 barrels 1.20/0.69 10cm 100 $64
3BF120-69-15 3 barrels 1.20/0.69 15cm 100 $87

Multibarrel Borosilicate Glass*
Without Filament
Catalog Number Number of Barrels In/Outside Diameter Overall Length Pieces per Package Price
3B100-75-10 3 barrels 1.00/0.75 10cm 75 $67
4B100-75-10 4 barrels 1.00/0.75 10cm 75 $70
* Multibarrel borosilicate requires a custom filament.
Please contact Sutter Instrument for more information when ordering.

Quartz Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
QF100-50-7.5 1.00mm 0.50mm 7.5cm 100 $132
QF100-50-10 1.00mm 0.50mm 10cm 100 $189
QF100-60-7.5 1.00mm 0.60mm 7.5cm 100 $132
QF100-60-10 1.00mm 0.60mm 10cm 100 $182
QF100-70-7.5 1.00mm 0.70mm 7.5cm 100 $152
QF100-70-10 1.00mm 0.70mm 10cm 100 $182
QF100-70-15 1.00mm 0.70mm 15cm 100 $200
QF120-60-7.5 1.20mm 0.60mm 7.5cm 100 $153
QF120-60-10 1.20mm 0.60mm 10cm 100 $189
QF120-90-10 1.20mm 0.90mm 10cm 100 $136
QF150-75-7.5 1.50mm 0.75mm 7.5cm 100 $136
QF150-75-10 1.50mm 0.75mm 10cm 100 $150

Quartz Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
Q100-30-7.5 1.00mm 0.30mm 7.5cm 100 $137
Q100-30-15 1.00mm 0.30mm 15cm 100 $144
Q100-50-7.5 1.00mm 0.50mm 7.5cm 100 $132
Q100-50-10 1.00mm 0.50mm 10cm 100 $176
Q100-70-7.5 1.00mm 0.70mm 7.5cm 100 $123
Q100-70-10 1.00mm 0.70mm 10cm 100 $176
Q120-40-7.5 1.20mm 0.40mm 7.5cm 100 $207
Q120-60-7.5 1.20mm 0.60mm 7.5cm 100 $182
Q120-90-7.5 1.20mm 0.90mm 7.5cm 100 $156
Q120-90-10 1.20mm 0.90mm 10cm 100 $200
Q150-50-7.5 1.50mm 0.50mm 7.5cm 100 $189
Q150-75-7.5 1.50mm 0.75mm 7.5cm 100 $132
Q150-75-10 1.50mm 0.75mm 10cm 100 $142
Q150-110-10 1.50mm 1.10mm 10cm 100 $207
Q165-115-10 1.65mm 1.15mm 10cm 100 $182

Solid Quartz Rod
Catalog Number Style Outside Diameter Overall Length Pieces per Package Price
QR100-7.5 solid 1.00mm 7.5cm 100 $85
QR100-10 solid 1.00mm 10cm 100 $85
QR100-15 solid 1.00mm 15cm 100 $85

Quartz Theta Glass
Catalog Number Outside Diameter Septum Thickness Overall Length Pieces per Package Price
QT120-90-7.5 1.20/0.9mm 0.15mm 7.5cm 50 $90

Multibarrel Quartz Glass
Catalog Number Number of Barrels OD/ID in mm Overall Length Pieces per Package Price
7Q033-16-10 7 barrel 1.00/[.33/.16ea] 10cm 50 $252

Aluminosilicate Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
AF100-64-10 1.00mm 0.64mm 10cm 100 $144
AF120-77-10 1.20mm 0.77mm 10cm 100 $144
AF120-87-10 1.20mm 0.87mm 10cm 100 $144
AF150-100-10 1.50mm 1.00mm 10cm 100 $144

Aluminosilicate Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package Price
A100-64-10 1.00mm 0.64mm 10cm 100 $123
A120-77-10 1.20mm 0.77mm 10cm 100 $123
A120-87-10 1.20mm 0.87mm 10cm 100 $123
A150-100-10 1.50mm 1.00mm 10cm 100 $123

Borosilicate Theta Glass
Catalog Number O.D/I.D. Septum Thickness Overall Length Pieces per Package Price
BT-150-10 1.50/1.17mm 0.165mm 10cm 100 $105

Solid Borosilicate Rod
Catalog Number Style Outside Diameter Overall Length Pieces per Package Price
BR-100-10 solid 1.00mm 10cm 250 $64
BR-100-15 solid 1.00mm 15cm 250 $69

US PRICES > Filaments and Accessories
International prices vary by country due to associated importation costs. Please contact your local distributor or Sutter Instrument for a quotation. Prices subject to change without notice.

FILAMENTS (Minimum purchase of filaments is 4)
Appropriate filament selection depends on your research application, but a general guideline for filaments is as follows:

Box Filaments are recommended for small to large diameter borosilicate glass, double barreled, or aluminosilicate glass. Box filaments are particularly suitable for patch pipettes, slice preparations, and for sharp electrodes and microinjection pipettes where gradual parallel walls would aid cell penetration. When using a box filament, the size of the square box should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass that you will be using.

For IVF and ICSI applications, a 2.5mm x 4.5mm box filament (FB245B) is recommended. For pronuclear injection work, we recommend a 2.5mm x 2.5mm box filament (FB255B). Call for more specific advice or to request a combination of settings, filaments, and glass for your application.

Trough Filaments are excellent general purpose filaments and are recommended for creating longer slice patch pipettes and sharp electrodes. The trough filament is not ideal for making patch pipettes or when using glass over 1.2mm OD. If very short tapers are required for patch electrodes or for microinjection, a 2.5mm or 3mm box filament is recommended.

Sutter pre-programs the P-97 with a 2.5mm x 2.5mm box filament unless an alternative filament is requested.

For either shape of filament (box or trough), increasing the filament width tends to increase the length of the pipette taper. If there are specific questions as to the sizes needed, please call and our technical support staff will guide you in your choice.

Trough filament Box filament
Loop filament Nichrome filament

Box Filaments P-1000, P-97, P-87, P80PC, PC-84, P-77B
Catalog Number Description Price
FB215B 2.0mm square box filament, 1.5mm wide $44
FB220B 2.0mm square box filament, 2.0mm wide $44
FB230B 2.0mm square box filament, 3.0mm wide $44
FB255B 2.5mm square box filament, 2.5mm wide $44
FB245B1 2.5mm square box filament, 4.5mm wide $44
FB315B 3.0mm square box filament, 1.5mm wide $44
FB320B 3.0mm square box filament, 2.0mm wide $44
FB330B 3.0mm square box filament, 3.0mm wide $44

Trough Filaments P-1000, P-97, P-87, P80PC, P80C, PC-84, P-77B
Catalog Number Description Price
FT315B 1.5mm wide trough filament $44
FT320B 2.0mm wide trough filament $44
FT330B 3.0mm wide trough filament $44
FT345B 4.5mm wide trough filament $44

P-30 Filaments
Catalog Number Description Price
PF30T15 1.5mm wide trough filament $44
PF30T20 2.0mm wide trough filament $44
PF30T30 3.0mm wide trough filament $44
PF30N2 Nichrome filament – 3 turns (BEFORE SN# P30-680) $44
PF30N-42 Nichrome filament – 4 turns (AFTER SN# P30-679) $44

P-77A Loop Filaments
Catalog Number Description Price
(SN# 160 and greater)
FL315A 3.0mm loop filament, 1.5mm wide $44
FL320A 3.0mm loop filament, 2.0mm wide $44
FL325A 3.0mm loop filament, 2.5mm wide $44
(SN# less than 160)
FL315X 3.0mm loop filament, 1.5mm wide $44
FL320X 3.0mm loop filament, 2.0mm wide $44
FL325X 3.0mm loop filament, 2.5mm wide $44

Accessories
Catalog Number Description Price
FILAMENT Custom platinum/iridium filament $65
FPS Fire polishing spacer for P-97, P-87, P-2000 pullers $29
FS1875 Platinum/iridium sheet, 18mm x 75mm x 0.05mm (0.002in) $388
CTS Ceramic tile for scoring glass $22
BX10 Pipette storage box - Holds 10 (4 3/4 x 3 5/8 x 3/4 in) $22
BX20 Pipette storage box - Holds 20 (7 x 3 5/8 x 3/4 in) $22

1 For P-87, please contact Sutter
2 P-30/N pullers (serial number lower than P-30-680) came with a P30N filament installed. You can now select either the P30N or P30N-4 filament. With the P30N-4 you can 1) use lower heat settings, 2) pull longer tapers (2-3cm) and 3) use aluminosilicate glass. If you need to pull very short tapers, the P30N is recommended.