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| P-97
Flaming/Brown
Micropipette Puller
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P E C S > P - 9 7 |
Dimensions
21in x 14in x 12in
53cm x 36cm x 30cm |
Weight
62 lbs
29 kg |
Electrical
115/230 volts
50/60 Hertz power line |
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The Model P-97 micropipette puller
is the latest generation of the Flaming/Brown type puller for fabrication
of micropipettes, patch pipettes and microinjection needles. While retaining
many of the features on the Model P-87, the P-97 offers improvements
in mechanical, electronic and software design. The result is better control
of the pulling process and a higher degree of reproducibility. The P-97
combines a proven mechanical system with a sophisticated, programmable
microprocessor controller. This programmable control of the pulling parameters
allows the investigator to design application specific pipettes from a
wide range of glass compositions and sizes.
| F
E A T U R E S > P - 9 7 |
| Environmental
chamber. |
| Programmable
air pressure. |
| Memory
storage for up to 100 programs. |
|
Write protection and date stamp for each program. |
| Two
symmetrical pipettes with each pull. |
| Preprogrammed
sample programs for intracellular and patch pipettes. Special programming
on request. |
| Ramp
test—self test for establishing program heat settings when a
new filament or glass is introduced. |
| Vacuum
fluorescent display. |
| Internal
memory test. |
| Constant
current power supply for filament and pull solenoid. |
| Looping
pull cycle for fabrication of patch type micropipettes. |
| Self-contained
air supply with filtration system and humidity control. |
| Consistent
and reliable electrodes with tip diameters less than 0.1µm. |
| Control
over the time and pressure at which the air is delivered. |
| Optimized
velocity sensing circuit for maximized sensitivity and reproducibility. |
| Quality
control, SEM photograph of a tip pulled with each puller; criterion
is tip measurement less than 0.1µm and typically is ~0.06µm. |
A number of new features have been incorporated in the
design of the P-97. One of the most apparent is the environmental
chamber which houses the area surrounding the heating filament. The environmental
chamber is designed to minimize the effect of changing humidity on the
reproducibility of pulled pipettes. A 25% increase in power over the previous
version allows for the use of larger heating filaments, larger diameter
glass and multi-barreled glass. The metal jaws that clamp the heating
filament have also been redesigned to minimize heat retention. A gas delivery
mode switch provides for extended cooling for large diameter and multi-barreled
glass. A spring-loaded clamping mechanism has been added for easier loading
of glass. A vacuum fluorescent display has been added that allows easy
viewing from any direction.
New software improvements on the P-97 include a display of the
total time that the heat is on to assist in program development and troubleshooting.
Up to 100 programs can now be written and stored in memory, which makes
the P-97 suitable for multiple users. These programs can now be write-protected,
adding security to prevent programs from being changed or altered inadvertently.
The display shows the last date and time the program was written or edited.
In addition, the air pressure is included as a programmable parameter.
The P-97 contains a ramp test to overcome the difficulties of program
adjustment when a new filament is installed or to help characterize a
new glass and filament combination. This test allows for the rapid adjustment
of heat values in established programs and provides estimates for establishing
heat settings when using new sizes or compositions of glass capillaries.
As with all Sutter instruments, the electronics have been carefully designed
to maximize noise rejection.
*Patent
No.4,600,424
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