P E C S > P - 2 0 0 0
30in x 14.25in x 13.25in
76cm x 36cm x 33.5cm
50/60 Hertz power line
1 LASER PRODUCT
The P-2000 micropipette
puller represents a significant advance in the technology of fabrication
of micropipettes, optical fiber probes, and nanospray tips. The P-2000
integrates a CO2 laser-based heat source with the
technology derived from our extensive experience with conventional pullers.
This system offers capabilities unmatched by other pullers.
E A T U R E S > P - 2 0 0 0
of pulling quartz, borosilicate and aluminosilicate glass.
programmable including heating filament characteristics.
laser has no melting point limit as with conventional metal filaments;
and therefore, cannot be burned out.
electrodes with tip diameters smaller than 0.03µm.
velocity sensing circuit for maximized sensitivity and reproducibility.*
life of the CO2 laser is expected to be in
excess of ten years with normal use, after which the laser can be
refurbished by the Sutter Instrument Company for a nominal charge.
programs can be write-protected in order to secure them from inadvertent
total time that the heat is on during the pull is displayed for improved
date and time stamp is displayed to show the last time that a program
has been changed.
P-2000/F is ideal for applications such as nanospray and NSOM.
sample programs for intra-cellular and patch pipettes.
The P-2000/F also comes with an NSOM tip program.
While the P-2000 is suitable for working with most
conventional glasses, its primary advantage is the ability to work with
quartz glass (fused silica). Quartz offers superior material properties
for a variety of research applications. Quartz is stronger than other
glasses and can facilitate penetration through tough tissues which would
normally break conventional pipettes(1) . For applications requiring a
low noise glass, users will find that quartz is the lowest noise glass
available(2,4). Quartz contains none of the metals used in conventional
glasses(3). Optically, quartz is virtually free from fluorescence when
A CO2 laser was selected as the
heat source for the P-2000 for several reasons. First, the nominal
emission wavelength of the laser approximates the resonant frequency of
the SiO2 lattice in glass. Thus,
quartz and other conventional glasses can be melted when the appropriate
laser power is supplied. Second, laser heat is clean and leaves no metal
residue on the pipette as do conventional heating filaments. Third, laser
heat can be turned off instantly, leaving no residual filament heat. Lastly,
the user can program the amount and distribution of heat supplied to the
The P-2000 can store up to 100 separate programs, with each program
consisting of up to 8 command lines. Programmable parameters include;
laser power level, scan width, trip velocity, delay/ laser on time, and
hard pull strength.
One important consideration for the use of the P-2000 is the diameter
of the glass used. The P-2000/G is designed to produce
even heating on glass up to 1.2 mm in outside diameter. Larger diameter
glasses can be used with the P-2000/G (up to 1.5 mm quartz and
1.8 mm conventional glasses), but the performance is best with glass that
is 1.2 mm diameter or less.
The P-2000/F works well with small diameter glasses such as optical
fibers, and with small diameter fused silica capillary commonly used for
the manufacture of nanospray tips. Small diameter glass (outer diameter
in the range of 0.125 mm to 0.6 mm) requires special puller bars as well
as an optical alignment optimized for the smaller diameter material.
As with larger diameter glass, a wide range of tip sizes and taper geometries
can be produced with the P-2000/F and small diameter glass. We
have drawn optical fiber tips ranging from less than 10nm to more
than 5µm. Please consult our technical staff for further information.
These references describe the Flaming/Brown pullers and contain valuable
information applicable to the P-2000.
(1) Munoz, J.L. and Coles, J. Quartz micropipettes for intracellular voltage
micro- electrodes and ion selective microelectrodes. Journal of Neuroscience
Methods: 22:57-64, 1987.
(2) Rae, J.L. and Levis, R. A. A method for exceptionally low noise single
channel recordings. European Journal of Physiology - Pfl.gers Archiv:
(3) Zuazaga, C. and Steinacker, A. Patch-clamp recording of ion channels:
Interfering effects of patch pipette glass. News in Physiological Science:
5:155- 159, 1990.
(4) Levis, R. A. and Rae, J. L. The use of quartz patch pipettes for low
noise single channel recording. Biophysical Journal: 65:1666-1677, 1993.