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XenoWorks™
Digital Microinjector
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P E C I F I C A T I O N S > |
DIGITAL
MICROINJECTOR
Hold Function
Hold Pressure: -350 to +350 hPa in 1hPa increments
Inject Functions
Inject Modes: Continuous or pulse
Inject Presure: 0 to 5600 hPa in 7hPa increments
Inject Time: 0 to 10 seconds in 0.1 second increments
Compensation Pressure: adjustable in 1hPa increments
Clear Pressure: Ramps from current Inject value to 5600 at 1000 hPa/sec.
Inject Command: Remote interface or foot switch
Transfer
Function
Transfer Pressure: -175 to +175 hPa in 1hPa increments
Maximum Pressure
5600 hPa (inject channel)
Controls
Tactile keys, rotary optical encoders 3 x 7-segment LED
display
Micropipette Holder
Diameter: 4mm
Accepts 1mm O.D. capillary glass
2m ETFE tubing included
Electrical
BRE110: 100-120 Volts, 60Hertz
BRE220: 220-240 Volts, 50Hertz
Dimensions
Size:
Compressor module
407mm x 440mm x 150mm,
User interface module
164mm x 123mm x 70mm
Weight:
Compressor module: 1504g, User interface module: 42g
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Designed
primarily for transgenic mammal workstations, the XenoWorks Digital
Microinjector is capable of holding oocytes and early embryos
immobile while simultaneously providing a separate pressure channel for
pronuclear microinjection of DNA or the gentle control of embryonic stem
cells as they are transferred into blastocysts.
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E A T U R E S > |
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DIGITAL MICROINJECTOR
Built-in compressor with remote user interface
Two independent pressure channels
Negative and positive pressure
Two injection modes: pulse and continuous
Four functions: Hold, Transfer, Inject, Clear
Transfer channel provides compensation pressure for high pressure injection
Four range settings for transfer/compensation pressure
Rotary controls for continuous pressure adjustment
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The microinjector
employs two independent pressure control channels. The self-contained
vacuum channel for gentle suspension-cell holding applications can be
switched on and off as needed, and a responsive rotary control is used
to dial the pressure up or down according to the degree of hold required.
A second channel is available which is capable of delivering both high-pressure
for solution injection, and gentle positive/negative pressure for transfer
of embryonic stem cells. Two modes of injection are available on this
channel; in Constant Flow mode, the inject pressure set is applied to
the micropipette tip for as long as the hand- or foot-switch is held
down. In Pulse mode, the inject pressure is applied for a precisely timed
duration,
which can be set in 0.1 second increments. Both injection duration and
pressure are easily selectable using rotary controls on the remote interface.
Optimal ergonomics are again a prime consideration; the remote control
unit provides access to all keys and rotary controls necessary to manipulate
the parameters in the two pressure channels, while the separate compressor
module can be shelf- or floor-mounted away from the microscope to eliminate
vibration interference.
The configuration of any microinjection system will depend
upon the application for which it is used. Some common applications and
system configurations are:
- Zygote pronuclear DNA microinjection
- Embryonic stem cell transfer into blastocyst
- Intracytoplasmic Sperm Injection (ICSI)
- Piezo-assisted ICSI
- Microinjection of cultured, adherent cells
- Somatic cell nuclear transfer
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