Products and Software > Microinjection > XenoWorks Digital Microinjector
XenoWorksDigital Microinjector

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S P E C I F I C A T I O N S >

DIGITAL MICROINJECTOR

Hold Function
Hold Pressure: -350 to +350 hPa

Inject Functions
Inject Modes: Transient or timed

Inject Presure: 0 to 5600 hPa in 70hPa increments

Injection Time:
0 to 10 seconds in 0.1 second increments

Inject Activation:
Hand or foot switch

Clear Pressure:
Ramps from current Inject value to 5600 at 1000 hPa/sec.

Transfer Function
Transfer Pressure: -175 to +175 hPa

Maximum Pressure
5600 hPa (inject channel)

Controls
Tactile keys, rotary optical encoders 3 x 7-segment LED display

Tubing 2m ETFE

Micropipette Holder
Diameter: 4mm
Accepts 1mm O.D. capillary glass

Electrical
BRE110: 100-120 Volts, 60Hertz
BRE220: 220-240 Volts, 50Hertz

Dimensions
Size:
Compressor module
407mm x 440mm x 150mm,
User interface module
164mm x 123mm x 70mm

Weight:
Compressor module: 1504g, User interface module: 42g

Designed primarily for transgenic mammal workstations, the XenoWorks Digital Microinjector is capable of holding oocytes and early embryos immobile while simultaneously providing a separate pressure channel for pronuclear microinjection of DNA or the gentle control of embryonic stem cells as they are transferred into blastocysts.

F E A T U R E S >

DIGITAL MICROINJECTOR

Four injection modes:
Hold, Transfer, Inject, Clear

Two independent pressure channels

Femtoliter resolution injection

Rotary controls for continuous pressure adjustment

Integral compressor with remote control pad


The microinjector
employs two independent pressure control channels. The self-contained vacuum channel for gentle suspension-cell holding applications can be switched on and off as needed, and a responsive rotary control is used to dial the pressure up or down according to the degree of hold required.

A second channel is available which is capable of delivering both high-pressure for solution injection, and gentle positive/negative pressure for transfer of embryonic stem cells. Two modes of injection are available on this channel; in Constant Flow mode, the inject pressure set is applied to the micropipette tip for as long as the hand- or foot-switch is held down. In Pulse mode, the inject pressure is applied for a precisely timed duration,
which can be set in 0.1 second increments. Both injection duration and pressure are easily selectable using rotary controls on the remote interface. Optimal ergonomics are again a prime consideration; the remote control unit provides access to all keys and rotary controls necessary to manipulate the parameters in the two pressure channels, while the separate compressor module can be shelf- or floor-mounted away from the microscope to eliminate vibration interference.

The configuration of any microinjection system will depend upon the application for which it is used. Some common applications and system configurations are:

- Zygote pronuclear DNA microinjection
- Embryonic stem cell transfer into blastocyst
- Intracytoplasmic Sperm Injection (ICSI)
- Piezo-assisted ICSI
- Microinjection of cultured, adherent cells
- Somatic cell nuclear transfer