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P E C S > P M M - 1 5 0 F
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Piezo Impact Drive Unit
(MB-U)
Moving range: approx. [plus or minus sign] 5mm
Traveling speed: 0.04mm/s max
Traveling resolution: approx. 0.1µm
Operation Box
Two adjustment dials: 16 speed levels, 16 intensity levels
FootSwitch
Arrow pedal to move left
Pedal selection indicator
Dimensions
Controller: 9.5in x 13in x 5.5in
240 x 340 x 140 mm
Operation Box: 4in x 6.3in x 2.4in
100 x 160 x 60 mm
Foot Switch: 5.5in x 5.5 in x 1.5in
145 x 140 x 38 mm
Weight
Controller: 5 kg (11.02 lb)
Peizo Drive Unit: 125g (0.28lb)
Operation Box: 530 g (1.17lb)
Foot Switch: 820 g (1.81 lb)
Electrical
AC supply of 100-240Volts
50/50Hz, 60mA.
Temperature
Operates at 5-40º C
Avoid high humidity
The PMM-150FU has been designed for multiple applications in micromanipulation
including:
Enucleation
Nuclear Microinjection
ICSI
ES Cell Injection
Assisted Hatching
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Sutter Instrument offers the PrimeTech PMM-150FU
piezo impact drive for challenging microinjection applications such as
mouse nuclear transfer, intracytoplasmic sperm injection (ICSI) and blastocyst
injection. Oocyte plasma membranes, particularly those of the mouse, are
particularly sensitive to microinjection, which tends to result in oocyte
death. Initially designed for porcine oocyte injection, the PrimeTech
PMM was adapted for use in mouse in 1995 (Kimura and Yanagimachi,
1995), and since then has become the gold standard for mouse nuclear transfer
and ICSI.
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E A T U R E S > P M M - 1 5 0 F U |
PRIMETECH
PIEZO IMPACT DRIVE
Smooth
insertion of microcapillary into elastic cell membrane
Precise movement of micromanipulator within range of travel
Inertial force from piezo-electric control minimizes damage of elastic
biomembranes
High resolution and precise positioning (minimum controllable dimensions)
Compact design |
The PMM-150FU harnesses the "piezoelectric
effect": the tiny, but extremely rapid distortion of a piezoelectric
crystal lattice when an electrical current is applied to it. This causes
the micropipette holder, which is placed in direct contact with the piezo
element to vibrate. Two modes of piezo "drilling" are available
with the PMM-150FU: A continuous train of pulses of desired frequency
and amplitude are used to vibrate the micropipette, gently penetrating
the zona pellucida of an oocyte or embryo, then a single pulse, again
of desired amplitude, is used to break the oolemma and inject the oocyte.
This approach makes it possible to achieve up to 100% survival of injected
oocytes.
Included:
XenoWorks Micromanipulator-specific micropipette holder clamp
PrimeTech factory warranty
Full technical support for peizo impact applications.
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