Products and Software > Imaging > Lambda DG-4 Plus
Lambda DG-4 Plus
/ DG-5 Plus
Ultra High Speed Wavelength Switcher
 

Recommended Accessories:
Microscope Adapters

Related Products:
SmartShutter™

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LAMBDA - DG-4 / DG-5 PLUS
Lamp Type
300 Watt pre-aligned, ozone free xenon arc bulb
(Full spectrum available upon request)
Output Range
330nm to 700nm - Ozone free
300nm to 700nm - Full spectrum
Lamp Lifetime
Expected lifetime 1,000 hours
(All bulbs have a prorated warranty for 500 hours)
Power Consumption
350 Watts
Filter Diameter
DG-4: Four, 25mm (1inch)
DG-5: Two, 25mm (1inch) and
Three 18mm
Dimensions
10in x 10in x 19in
25cm x 25cm x 48cm
Light Guide
2 meters long
3mm diameter
Weight
45lbs
20kg
Electrical
115/230 Volts
50/60 Hertz power line
RoHS Compliant

The Lambda DG-4/DG-5 Plus is a complete illumination system offering speed and versatility for experiments requiring rapid wavelength switching. The instrument retains all the advantages of interference filter based systems, yet eliminates the temporal constraints imposed by traditional filter changing devices like filter wheels. Switching between any two wavelengths is achieved in less than the 1.2msec vertical retrace period of a video signal, allowing the user to perform real-time video imaging. The high switching speed of the Lambda DG-4/DG-5 Plus facilitates the ability to follow fast changes in ion concentrations in dual wavelength ratio imaging applications and to monitor other concomitant changes in the studied system at additional wavelengths.

F E A T U R E S > LAMBDA DG-4 / DG-5 PLUS

COMPLETE SYSTEM FOR WAVELENGTH SWITCHING

The Lambda DG-4 / DG-5 Plus is a complete integrated 300 Watt light source and wavelength switching excitation system.

Four or five interference filters can be easily installed in the DG-4 or DG-5 Plus, respectively. An additional standard neutral density filter can be inserted in the common path of the light.

The light guide output from the Lambda DG-4 / DG-5 Plus provides uniform spatial illumination, as well as vibration isolation from your microscope.

A cold mirror assembly eliminates IR radiation, extending the lifetime of the optics and the light guide and preventing the exposure of the sample to IR when white light is necessary.

INTEGRAL SHUTTERING

The Lambda DG-4 /DG-5 Plus provides a high speed shutter function with open/close times of 500µs. The shutter function reduces light intensity by 5 to 6 orders of magnitude.

If complete light shuttering is required, a mechanical shutter (i.e. SmartShutter™) can be incorporated into the Lambda DG-4 / DG-5 Plus.

INTEGRAL NEUTRAL DENSITY FILTERING

Neutral density filtering is achieved by offsetting the output galvanometer such that light is not centered on the liquid light guide. Up to 15 logical filters can be defined with this method. Due to the scrambling effect of the light guide, the output has excellent uniformity.

Direct insertion of neutral density optical filters is also possible in the filter holders at any of the four optical channels. A final neutral density optical filter can be placed in the exiting light path which will reduce the light output from all optical channels.

TWO OUTPUTS FOR MONITORING FILTER POSITION
A 4 bit TTL signal transmits the current optical channel (filter) position.

A digital-to-analog converter (DAC) output produces a voltage showing which filter is in use.

TURBO BLANKING
The turbo blanking feature is designed for situations when the switching is done between non-adjacent filter positions. When the instrument is in this mode, the input and output galvanometer mirrors move out of sync, thus preventing the sample from being exposed to light of unwanted wavelength during switching and shuttering.

DIRECT COMPUTER CONTROL
Computer control of the instrument is possible via parallel or serial port.

Narrow bandpass systems, such as single cavity interference filters, grating monochromators, and A.O. modulators, pass unwanted harmonics of the desired wavelength. Also, with variable wavelength devices, it is not always possible to obtain sufficient blocking of out-of-band wavelengths. Modern interference filters, as used in the Lambda DG-4/DG-5 Plus, do not pass harmonics and have integral blocking characteristics 1000 times better than typical monochromator systems. For the same full width at half maximum (FWHM), interference filters have a narrower spectral bandpass due to the absence of the slit function.
The dual galvanometer design of the Lambda DG-4/DG-5 Plus allows tuning of the relative intensities at each wavelength. This adjustment, in variable wavelength devices with a single optical path, is difficult to obtain. Unlike monochromators and other wavelength selective systems, the Lambda DG-4/DG-5 Plus can be used as a source of white light when required.

How it works:
This unique optical design of the Lambda DG-4/DG-5 Plus is based on dual scanning galvanometers utilizing interference filters for wavelength selection. The light from the xenon arc lamp is focused on the first galvanometer mirror which directs it via a parabolic mirror, through one of the interference filter channels. Following the filter, a second parabolic and a second galvanometer mirror collect and redirect the light into the light guide. A cold mirror in the beginning of the light path eliminates the IR radiation, reducing significantly the amount of heat absorbed by the optics and the sample. Cold mirrors modified to pass near-IR to 780 to 880nm are also available.
A built-in shutter function allows reduction of light intesity by six orders of magnitude. For applications requiring mechanical shuttering, like time lapse experiments or very sensitive samples, the SmartShutter™ can be integrated into the device to assure that the light is completely blocked.

The standard system, the Lambda DG-4 Plus, holds up to four 25mm interference filters. The Lambda DG-5 Plus, a five filter version, accommodates three 18mm and two 25mm filters. While the switching time between any two wavelengths is done in less than 1.2msec, the dwell time at any wavelength is arbitrarily set by the user. The Lambda DG-4/DG-5 Plus provides 0.5msec switching times between adjacent filter positions and 20% greater light output.

The light guide can be coupled to the illumination port of most microscopes using an optional adapter.